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    The Assisted Laser Hatching is an additional lab technique designed to improve the efficiency of procedures such as IVF & ICSI. Also other Assisted Reproductive Technology. The unfertilized egg is surrounded by a shell called zona pellucida. The zona has an important role in fertilization as it allows only one sperm to penetrate the zona and enter the egg to achieve fertilization. After fertilization of the egg, during the cleavage stage of an embryo, zona pellucida hardens. This development is normal and the purpose is to keep the cells in the egg together. The embryos have to “hatch” or break out of the zona in order to embed into the endometrial lining of the uterine cavity. This occurs about 2-3 days after embryo transfer when the embryo is at the blastocyst stage. Naturally, this takes place by expanding/contracting of the zona until it breaks, allowing the blastocyst to “hatch”. When sperm and eggs are cultured in IVF laboratory, the zona pellucida hardens at a much faster rate than natural, making it more difficult for the embryo to implant. Prior to implantation, the embryo has to escape out of its zona pellucida by a process known as hatching. If this process is not completed properly, implantation failure occurs and a pregnancy cannot be achieved.
    Assisted hatching is the process of creating a hole in zona pellucida, to aid the embryo in the hatching process
    At Anmol Fertility Clinic, we offer the Laser technology for Assisted Hatching (LAH), where a precision laser beam is focused over the zona pellucida making a small opening, between 10-20 microns to facilitate embryo hatching. LAH is done just before the Embryo Transfer. This helps to increase pregnancy rates by improving implantation rates since embryo hatching is facilitated. A fast, safe, simple method compared to the old method of hatching which was performed by using an acidic medium.

When is Assisted Laser Hatching recommended?

  • With day 3 embryos having a thick Zona
  • With previously failed IVF/ICSI cycles
  • For women older than 37 years of age, having a tendency to produce eggs with a harder zona pellucida than younger women
  • With a high Follicle Stimulating Hormone (FSH) level
  • If undergoing IVF/ICSI treatment for the first time, who are considered poor responders because they have required a high dosage of gonadotrophins for poor response of ovaries.
  • Who in an earlier IVF cycle had low fertilization rate, resulting in three or fewer embryos for achieving fertilization
  • Who request laser assisted hatching and are fully informed of its use and benefits


    Blastocyst Culture & Transfer technique was developed to aid In-Vitro Fertilization. Traditionally embryos were transferred to the uterus on the third day after fertilization. Healthy embryo called “Blastocyst” is grown in the laboratory for 5 to 6 days after egg collection and insemination.
    In blastocyst culture, over the period of 5 to 6 days embryo gets divided into multiple cells. Usually, the strongest embryos make it to blastocyst stage as they have survived key growth and division processes and have a better chance of implanting once transferred. Central cells, also known as inner cell mass gives rise to the fetus after implantation, whereas the single layer cells outside blastocyst form trophectoderm that gives rise to the placenta.
    The real advantage of blastocyst transfer is the high birth rates. This technique is also intended towards minimizing the chance of multiple pregnancies.

Who Can Go For Blastocyst Culture & Transfer?

  • This technique is not for all IVF patients, it is usually for patients young at age with enough eggs available for retrieval.
  • Patients who had failed attempts to conceive.
  • For patients who are concerned about multiple pregnancies.

  • Anmol Fertility Clinic is one of the few infertility clinics in Jaipur to provide blastocyst culture as it requires advanced technology and excellent lab environment. We provide each and every level involved in blastocyst culture and Anmol Fertility Clinic has such advanced lab facility.


    To achieve pregnancy, an infertile woman can also opt for Egg Donation. At Anmol Fertility Clinic, both the donor and the recipient are made to understand the procedure and are given psychological counseling prior to an egg donation treatment cycle. The treatment cycle consists of having the donor go through hormone therapy for ovulation stimulation while the recipient’s uterus is synchronized to prepare for the embryo transfer. egg donation in jaipur By using IVF technology, our fertility specialist retrieves the eggs from the ovaries of the donor, which are then either fertilized by the sperm of the recipient’s male partner or through a sperm donor. The resulting embryo(s) are then placed into the recipient’s uterus.
    The donor’s contribution is to provide the genetic material, but the pregnancy (gestation), labor and birth, and parenting fall to the recipient. If pregnancy is achieved, the resulting child will be genetically related to the recipient’s partner if his sperm was used, but not to the recipient.
    Infertile couples have to consider various things when egg donation is the only option for them. They should undergo counseling and should patiently think about the implications of their decision. At this stage, no decision should be taken under emotional pressure.

Women may need egg donation in following circumstances

  • Poor egg quality
  • Unexplained infertility
  • Congenital absence of eggs or ovaries
  • Prior cancer treatments
  • A high risk of transmitting genetic diseases
  • Delay in starting a family
  • Recurrent abortions and IVF failures
  • High risk of passing genetic disorder to the baby


    A couple’s infertility or subfertility problem may be due to a combination of factors and may affect both man and woman. If standard IVF treatment fails or offers very small chance of success, the transfer of a donor embryo into the woman’s womb may be the only way to conceive. Embryo Donation is for couples struggling with infertility in which eggs and sperms are absent. It is a cost-effective, well established and successful technique for conceiving.
    The Success rate of Embryo Donation is more than 65%. It is more successful than egg donation and has fewer medical complications.


    At Anmol Fertility Clinic, embryo donation is advised to:
  • Infertile couples
  • Female whose ovaries do not produce eggs because of premature menopause
  • Female who has undergone cancer or endometriosis treatment
  • Woman with non-functioning ovaries
  • Couples who have experienced repetitive IVF failure
  • Male partner with poor or zero sperm count

Common reasons for performing icsi

    At anmol fertility clinic, the common reasons for icsi are:
  • fertilisation failure in previous ivf cycle
  • poor fertilization in previous ivf cycles
  • low sperm count
  • poor sperm morphology
  • poor sperm motility
  • when the frozen sample to be used
  • when pre genetic implantation diagnosis is warranted.

How is icsi performed?

    Icsi technique is mainly used for the male counterparts experiencing repeated ivf failures due to serious fertility issues. it is a very effective method used to fertilize eggs have been aspirated from the females.
  • icsi technique involves the use of tools and equipment with a small inverted microscope that enables the embryologist to select and collect healthy individual sperm in a specially devised icsi needle.
  • this needle is carefully advanced into the outer shell of egg membranes and the sperm is injected into the cytoplasm (inner part of the egg).
  • icsi usually results in fertilization in about 75% - 80% of the cases
  • however, initially, the female is stimulated with medications for egg retrieval procedure so that several eggs are obtained for this procedure.


    ICSI: Intra-Cytoplasmic Sperm Injection The Intra-cytoplasmic sperm injection, commonly known as ICSI is basically an Assisted Reproductive Technology (ART). This technology is used to solve issues related to male infertility as a result of no sperm ejaculating in the semen. The fertilization stage of in-vitro fertilization or IVF is strengthened as a result of the injection of a sperm into a mature egg. This fertilized egg is then inserted into the uterus of a woman. At Anmol Fertility Clinic, our doctors may advise ICSI to males with low sperm count and/or low sperm motility even after the repeated success of IVF.


    At Anmol Fertility Clinic, the common reasons for ICSI are:
  • Fertilisation failure in previous IVF cycle
  • Poor fertilization in previous IVF cycles
  • Low sperm count
  • Poor sperm morphology
  • Poor sperm motility
  • When the frozen sample to be used
  • When Pre genetic implantation diagnosis is warranted.

Anmol fertility clinic may recommend ivf treatment

  • diagnosed with unexplained infertility
  • damaged or blocked fallopian tubes due to patients previous ectopic pregnancy/ hydrosalpinx/ post family planning operation
  • suffering from poor ovarian reserve
  • diagnosed with endometriosis
  • other techniques such as fertility drugs or intrauterine insemination (iui) have not been successful
  • male partner has fertility problems but not severe enough to undergo intra-cytoplasmic sperm injection (icsi)
  • donated eggs or your own frozen eggs are being used in the treatment
  • embryo testing is being used to avoid passing on a genetic condition to your child

Best timing for the treatment ?

    The iui procedure must take place when an egg or eggs are present, that is, during a woman’s fertile period. the best timing for iui would be about 36 hours after an hcg injection is administered to stimulate ovulation. the timing of iui may even be based on a natural lh surge which triggers ovulation. in such case, treatment is scheduled between 24 to 36 hours. this is because the egg does not survive for much longer than 24 hours after it is released unless it is fertilized.

Post Care for IUI Treatment

    Most women do not require special post-treatment care but if you suffer cramps or feel uneasy after the treatment, it makes sense to take it easy for a while. Some people reduce their aerobic activity and avoid lifting weights during this stage to increase chances of implantation. A pregnancy test is conducted 15 days after the treatment is performed and is evaluated by our best IUI doctors in Jaipur. Maintaining a positive frame of mind will really help you during this time.


    Sperm banking or Semen Banking is the term used for collection and storage of semen, a fluid containing sperms.

How long a sperm can be stored?

    Sperm can be stored indefinitely. There are reports of normal pregnancies from frozen sperm stored even for 12 years. However, each person’s sperm reacts differently to the freezing procedure.

What is the procedure of SEMEN BANKING?

    At Anmol Fertility Clinic, a donor is screened for medical fitness before collecting semen from him. The semen thus obtained is either used immediately for insemination or is stored at -1960C for six months. After 6 months the donor is retested for any infection, particularly for HIV. If the tests are negative, the sample is released from quarantine for insemination.
    The success rate of both fresh, as well as frozen semen, is almost similar. However, in the case of a stored sample, motility decreases rapidly within 12 hours, in contrast to fresh insemination, where the sperms stay alive in the body for 48-72 hours. The major advantage of frozen samples is its minimal risk of HIV infection.

What is Surrogacy?

    In surrogacy a woman carries the baby for nine months and then hands over the baby to the genetic parents who were unable to conceive. With IVF treatment new embryos are generated from the genetic couple. These embryos are then implanted in the surrogate host.
    Anmol Fertility Clinic offers a surrogacy program to couples as well as single parents that desirous of parenthood.
    We have a large data bank of surrogates who have been thoroughly screened for any infectious disease as well as any psychological disorders. All the potential surrogates are counseled at the clinic along with their husband/partner and are made to understand the entire process before they are enrolled as potential surrogates.
    Our nursing staff regularly makes monitoring visits to the surrogates during the nine months of pregnancy to ensure they are taking healthy diet and medicines regularly. Our surrogacy program thus enjoys a high success rate.
    Note: In surrogacy, there is no genetic relation between the child and the surrogate.

Who is surrogacy recommended for?

  • Females who are born with congenital absence of the uterus.
  • Female whose uterus was removed due to cancer or heavy menstrual bleeding.
  • Women who face medical conditions like hypertension or are diabetic which makes pregnancy risky for the women as well as the child.
  • Women whose uterus is deceased due to Ashermann’s Syndrome/Tubercular Endometriosis.


    In men, sometimes the sperms are absent in the semen. This could be due to either the sperms are not produced by the testis or due to outflow obstruction. If azoospermia is due to outflow obstruction then we can retrieve sperms directly from the testis by a procedure termed TESA/PESA.


    Testicular Sperm Aspiration (TESA)
  • TESA is performed on patients suffering from Obstructive azoospermia.
  • In TESA procedure sperm is directly extracted from the testicles by passing a fine needle directly into the testicle.
  • The retrieved sperm then can be injected into the woman’s oocytes by ICSI to form an embryo.
  • Before performing TESA, tests are done to check whether sperms are present or can be obtained at the biopsy.
  • Sperms extracted by TESA can be frozen and used later.


    Percutaneous Epididymal Sperm Aspiration (PESA)
  • PESA procedure is usually performed under sedation with pain relief, or else can also be done under general anesthetic
  • In some men, sperm can be extracted directly from the epididymis by passing a fine needle through the skin of the scrotum and placing it in the epididymis
  • Small amounts of fluid are collected and then examined for traces of sperm
  • The retrieved sperm is then prepared and then injected directly into eggs using ICSI technique.

Who might benefit from TESA/PESA?

    If there is a condition where men produce sperms but are unable to reach ejaculation due to
  • The absence of vas deferens (tubes that carry the sperm)
  • Blockage of vas deference secondary to any infection.
  • Other conditions like low sperm count or motility